Welcome to our interactive web interface which allows you to access the single-cell mRNA Seq data from 'Effector differentiation downstream of lineage commitment in ILC1 is driven by Hobit across tissues' by Friedrich et al.
We have analyzed hepatic ILC1 from Hobit-sufficient (Hobit Tom/WT) versus Hobit-deficient (Hobit Tom/KO) mice (described by Behr et al., Nature Immunology 2020), which are here abbreviated as WT and KO, respectively.
ILC1 were sorted as Lin- (CD4-CD5-CD8-CD19-TCRb-TCRgd-F4/80-Ly6G-Ter119-) NK1.1+ NKp46+ TdTomato+ cells, marked with Total-Seq antibodies (2 (A0302) for WT, and 1 (A0301) for KO), and one shared sequencing library was generated using the 10x chromium platform.
Cell clustering was performed using R and Seurat (v3.2.1). Trajectory analysis was performed using Slingshot (1.4.0)
Contact:
Würzburg Institute of Systems Immunology
Max Planck Research Group
Julius-Maximilians-Universität Würzburg
Versbacher Straße 9, D-97078 Würzburg
Email:
systemimmunologie@uni-wuerzburg.de
https://www.med.uni-wuerzburg.de/en/systemimmunologie/research/tissue-resident-lymphocytes-gasteiger-lab
RNA seq data from WT mice only
RNA seq data from WT and KO mice
RNA seq expression in WT vs KO
Expression of selected genes along pseudotime (combined WT and KO samples)